human normal hepatocytes hl7702  (ATCC)

Journal: BioMed Research International

Article Title: Partial Hepatectomy-Induced Upregulation of miR-1907 Accelerates Liver Regeneration by Activation Autophagy

doi: 10.1155/2018/3817057

Figure Lengend Snippet: miR-1907 promotes hepatocyte proliferation in vitro and in vivo . miR-1907 were overexpressed in CCL-9.1 (a) or in HL7702 (b) using miR-1907 mimics, and cell proliferation was assessed using the CCK-8 assay. miR-1907 were overexpressed in CCL-9.1 (c) or in HL7702 (d), and cell proliferation was assessed using EdU immunofluorescence staining. miR-1907 were overexpressed (e) or inhibited (f) in CCL-9.1, and cell proliferation was assessed using BrdU ELISA. (g) Immunohistochemistry analysis for PCNA showed the differences in hepatocyte proliferation between mice injected with miR-1907 and control. Original magnification ×200. Quantification of PCNA-positive cells at different timepoints after 2/3 PH was showed in (h). n=5. (i) Mice were injected with miR-1907 or control through the tail vein before and after 2/3 PH. The liver mass to body weight ratio was then calculated at different timepoint. ∗p <0.05.

Article Snippet: Mouse normal hepatocyte lines (CCL-9.1) and human normal hepatocyte lines (HL7702) were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: In Vitro, In Vivo, CCK-8 Assay, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Injection, Control

Journal: BioMed Research International

Article Title: Partial Hepatectomy-Induced Upregulation of miR-1907 Accelerates Liver Regeneration by Activation Autophagy

doi: 10.1155/2018/3817057

Figure Lengend Snippet: miR-1907 increases the activation of hepatocyte autophagy . (a) Western blot analysis of LC-3B expression in liver tissues at different timepoints after 2/3 PH. CCL-9.1 cells (b) or HL7702 (c) cells were treated with miR-1907, and the autophagosome formation was visualized by assaying activated green puncta. Punctate staining is indicative for the redistribution of LC3 to autophagosomes. The average number of green puncta per cell with standard deviation for each group is presented. Scale bar, 50 μ m. CCL-9.1 cells (d) or HL-7702 (e) cells were treated with miR-1907 and then western blot analysis of LC-3B protein expression to evaluate autophagy. CCL-9.1 cells (f) or HL7702 (g) cells were treated with miR-1907 and then western blot analysis of p62 protein level to evaluate autophagy. ∗p < 0.05.

Article Snippet: Mouse normal hepatocyte lines (CCL-9.1) and human normal hepatocyte lines (HL7702) were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Activation Assay, Western Blot, Expressing, Staining, Standard Deviation

Journal: Oncology Letters

Article Title: Atmospheric particulate matter 2.5 promotes the migration and invasion of hepatocellular carcinoma cells

doi: 10.3892/ol.2017.5947

Figure Lengend Snippet: PM 2.5 inhibits proliferation and induces apoptosis in HL7702 cells. (A) PM 2.5 inhibited proliferation and (B) induced apoptosis in a dose-dependent manner. *P<0.05 vs. the control group. PM 2.5 , particulate matter with an aerodynamic diameter ≤ 2.5 µm.

Article Snippet: The human HCC cell lines HepG2 and HuH-7, and human normal human HL7702 hepatocytes were purchased from the China Center for Type Culture Collection and were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin (all Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) in a humidified incubator with 5% CO 2 at 37°C.

Techniques: Control